For Analysis applications, RT‐qPCR is usually used not just to find out the existence or absence of an RNA but to evaluate its concentration. In such cases, a calibration curve need to be included in every single experiment, consisting of at least 6 serial dilutions of the in vitro−transcribed RNA https://www.facebook.com/NewTechFind/posts/pfbid02FCJT8WjvgB8mNgNjQTCGa79StJCpF6BAoHDqcZkpHnEqaVkXqXfgRZQTWycL4Whel